Detection of Serum Alkaline Phosphatase Isoenzymes with Phenolphthalein Monophosphate Following Cellulose Acetate Electrophoresis
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چکیده
منابع مشابه
Detection of serum alkaline phosphatase isoenzymes with phenolphthalein monophosphate following cellulose acetate electrophoresis.
Serum containing normal and abnormal levels of alkaline phosphatase activity were assayed for total enzyme activity, then fractionated by electrophoresis on cellulose acetate membranes for 20 mm. The new substrate, phenolphthalein monophosphate, was employed to locate the isoenzymes on the cellulose acetate membranes and to measure their activity by eluting and scanning procedures. The sensitiv...
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The isoenzymes of alkaline phosphatase (AP) in the serum of 30 normal subjects and 90 patients with common hepatobiliary disorders are distinguished using electrophoresis on cellulose acetate. Physicochemical profiles of these mixtures of serum isoenzymes are presented using thermostability and organspecific inhibitors (urea, L-phenylalanine, and L-homoarginine). The findings are reviewed and t...
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The manual procedure of Babson et al. (1) for the determination of serum alkaline phosphatase has been automated. The assay is based on the colorimetric determination of phenolphthalein formed on hydrolysis of phenolphthalein monophosphate. The procedure utilizes 0.16 ml. of serum without dialysis. Blanks are required only for turbid sera. Results are compared with those obtained by the manual ...
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We investigated the prevalence and characteristics of intestinal alkaline phosphatase (ALP; EC 3.1.3.1) identified in human serum by cellulose acetate electrophoresis in 8% of fasting serum samples from hospital patients (n = 500) and in 35% of fasting serum samples from patients with diabetes mellitus (n = 106; not differentiated between types 1 and 2). The intestinal ALP electrophoretic band ...
متن کاملPhenolphthalein monophosphate as a substrate for serum alkaline phosphatase. An appraisal.
A rapid and precise procedure for serum alkaline phosphatase employs phenolphthalein monophosphate as the substrate. This compound is readily hydrolyzed and directly provides a chromogen for colorimetric determination. The color produced is linear with respect to both enzyme concentration and incubation time and is stable. Reagent and serum blanks are negligible. Various factors which affect al...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1968
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/14.1.47